THE INFLUENCE OF SUBSTRATE AND ELECTRON ACCEPTOR AVAILABILITY ON BIACTIVE ZONE DYNAMICS IN POROUS MEDIA

Examination of the spatial and temporal distribution of biologically active zone in response to local changes in substrate and electron acceptor availability. Two methods are developed: monitoring of substrate and dissolved oxygen and in situ reporter gene activity in the column system. Materials and methods, solutions and sorbent, bacterial strain with a multi fibre optic luminescence detection system. Quantification of luminescence and substrate and dissolved oxygen concentrations. Hydrodynamic characterisation of the system and spatial variability of substrate biodegradation. Salicylate , naphthalene and pentafluorobenzoic acid breakthrough curves obtained from sterile column experiments and after a microbial activity throughout the bioactive zone with different salicylate input concentrations. Naphthalene breakthrough curves obtained from all sampling locations and luminescence emitted by bacteria P. putida during naphthalene degradation.